The Bottom Line

The BioFlo 120 is the bridge between iterative R&D and manufacturing readiness. It is the fastest and most cost-effective way to generate a robust data package and ensure your process is ready for pilot-scale.

Eppendorf BioFlo 120 bench-scale fermenter setup at Growth Curve Bio.
The Eppendorf BioFlo 120 bench-scale fermenter at Growth Curve Bio, run in parallel for fast control-versus-experimental comparisons.

At a Glance

  • Equipment TypeBench-scale, autoclavable fermenter
  • Working Volume0.8 to 2.2 L
  • ControlsTemperature, pH, dissolved oxygen
  • AdditionsUp to 4

Use Case

Process de-risking & scale-up validation

Growth Curve Bio employs the BioFlo 120 to facilitate efficient technology transfer and upstream process development. Through parallel bench-scale fermentation runs, the team rapidly evaluates control versus experimental conditions and validates process reliability before advancing to pilot-scale operations. This approach optimizes both timeline and budget allocation for clients.

Why BioFlo 120?

  • Operational flexibility. Suitable for batch, fed-batch, and fill and draw processes.
  • Precise control. 4 peristaltic pumps enable complex, highly controlled feeding and inducing strategies.
  • Real-time data. Industry-standard sensors constantly monitor temperature, pH, dissolved oxygen, and foam.

Key Inputs

  • Culture. Client-provided strain or cell line.
  • Media. Defined or complex media recipe.
  • Operational parameters. Setpoints for temperature, pH, and dissolved oxygen, along with the dissolved oxygen cascade (agitation, aeration, supplemental gas).

Key Outputs

  • Precise data. High-resolution logs of temperature, pH, dissolved oxygen, and components of the dissolved oxygen cascade.
  • Analytical material. Fermentation broth for offline analytics (at GCB, client site, or third-party lab).
  • DSP material. Fermentation broth for bench-scale downstream processing.
  • Scalable protocol. Recipes and control logic that are ready for pilot-scale vessels.