Our Process

Inside the Full-Stack Process

A precise look at what happens at each stage: the inputs we take in, the work we do, the equipment we run, and the data you receive. Built for your success.

Modular by stage Enter at any point Hand-off ready Recursive where it counts
(01) Overview

A modular process, not a fixed conveyor belt. Every stage produces a real, usable deliverable, so a program can enter where it makes sense and exit the moment it has what it came for.

Not every program goes through the entire cycle. A team that already has a validated strain can start at process optimization. A team that only needs a feasibility answer can stop after prototyping with a decision-grade data package in hand. There is genuine value at every hand-off point.

Some stages are also recursive by design. Prototyping and process optimization run as iterative design-build-test loops that repeat against quantitative targets until the data says the program is ready to move forward.

Enter at Any Stage

Bring us a target, a strain, or a working process. We meet the program where it is and pick up from there.

Hand-Off After Any Stage

Each stage ends with a deliverable you own. Take it and continue in-house, or carry on with us to the next stage.

Recursive Loops

Select stages iterate against quantitative targets. Fitting the biology to real production equipment is a loop: we measure, tune the process and the strain, and repeat until your endpoint is achieved.

(02) The Flow, End to End

One Chain, Five Stages, Many Valid Paths

From a raw target on the left to validated material and data on the right. Jump to any stage below to see its inputs, equipment, and outputs in full.

Enter at any stage ยท Hand off after any stage
Input

Your target molecule, pathway, strain, or existing process.

  1. 01

    Strain Engineering

  2. 02

    Rapid Prototyping

  3. 03

    Process Optimization

  4. 04

    Pilot / T&E Production

  5. 05

    Manufacturing Hand-Off

Output

Validated strains, a defined process, pilot material, and the data behind it.

(03) Every Stage in Depth

Inputs, Work, Equipment & Outputs

What we take in, what happens inside the stage, what we run it on, and exactly what you walk away with.

01

Strain Engineering

Design and build a production host engineered to express your target reliably under real fermentation conditions, not just in a shake flask.

Inputs
  • Target molecule or pathway (sequence, structure, or reference)
  • Quantitative performance targets: titer, purity, activity
  • Host constraints or preferences (regulatory, IP, GRAS status)
  • Any existing strains, constructs, or prior data
What We Do

We select the right microbial chassis for your molecule and engineer it to express: pathway and construct design, codon optimization, genetic stability, and tolerance to the real stresses of fermentation. Because we own every downstream stage, we design for commercial production from the very first edit and iterate the build against defined targets.

Equipment & Methods
  • Molecular cloning and DNA assembly
  • Transformation and high-throughput clone screening
  • Genotyping and sequence verification
  • Cryo-banking of validated production strains
Outputs & Data
  • Validated production strain with full sequence records
  • Construct maps and strain lineage documentation
  • Baseline expression data against targets
  • Banked, ready-to-scale strain
Hand-off point: take a documented, banked strain and run it yourself, or carry it straight into prototyping.
02

Rapid Prototyping

Prove the strain and molecule perform before committing to a longer, costlier program.

Inputs
  • Candidate strain(s) or construct(s)
  • Target performance and an analytical spec
  • The make-or-break feasibility questions to answer
  • Any reference material or comparator
What We Do

Candidate molecules move through fast, structured design-build-test cycles: small-scale expression, screening, and analytical characterization. The loops are tuned to answer feasibility first, so you get an early, honest read on whether a molecule performs and can be reproduced before capital is committed.

Equipment & Methods
  • Microplate and shake-flask cultivation
  • Parallel, structured screening workflows
  • Analytical characterization (chromatography, spectroscopy)
  • Reproducibility and replicate testing
Outputs & Data
  • Decision-grade feasibility data package
  • Screening and reproducibility results
  • A confident go / no-go recommendation with evidence
  • Ranked shortlist of candidates to advance
Hand-off point: stop with a feasibility and go / no-go package for your board or investors, or advance the winner to process optimization.
03

Process Optimization

Develop a reproducible, cost-aware bioprocess that meets your specifications and translates onto industrially representative equipment.

Inputs
  • Selected strain and target analytical spec
  • Cost-of-goods and scale targets
  • Any existing process baseline to improve on
  • Constraints on media, feedstock, or downstream
What We Do

We develop and refine the bioprocess itself (media and feed strategy, fermentation parameters, and downstream recovery), driving titer, yield, and batch-to-batch consistency up while pulling cost of goods down. Every change is measured against a defined analytical baseline and chosen to translate onto larger equipment, where some parameters scale linearly and others do not.

Equipment & Methods
  • Fully instrumented benchtop bioreactors (temperature, pH, DO, feed)
  • Design-of-experiments driven optimization
  • At-line and in-line process monitoring
  • Downstream unit ops: centrifugation, filtration, chromatography, drying
Outputs & Data
  • Defined, documented process with control ranges
  • Titer, yield, and cost-of-goods model
  • Analytical baseline and batch records
  • Scale-up risk assessment
Hand-off point: take a defined process and cost model to a manufacturer of your choice, or move into pilot production with us.
04

Pilot & T&E Production

Produce scale-representative material at 100 L and under, under defined controls, for your own testing, evaluation, and validation.

Inputs
  • A defined process and its control strategy
  • Material quantity and quality requirements
  • Your testing, evaluation, or validation plan
  • Any documentation standards to meet
What We Do

We produce your molecule at pilot scale, in batches at 100 L and under, under defined process controls. Pilot runs are where scale-up risk actually shows itself, so we put real material and real data in your hands and document each run so it carries cleanly into a regulated manufacturing environment later.

Equipment & Methods
  • Pilot-scale bioreactors at 100 L and under
  • Downstream recovery and purification at pilot scale
  • Quality-control and release analytics
  • Defined process controls with run documentation
Outputs & Data
  • Scale-representative material for your program
  • Full analytical and batch documentation
  • Process performance data at pilot scale
  • A validation-ready data package
Hand-off point: take pilot material and its data package for your own testing and validation, or continue into a managed manufacturing hand-off.
05

Manufacturing Hand-Off & Management

Package the process for scale and, if you want it, manage the transfer into commercial manufacturing as your technical advocate on the floor.

Inputs
  • A validated pilot process and documentation
  • Target CDMO or facility (or we help you select one)
  • Commercial volume and timeline targets
  • Quality and regulatory expectations
What We Do

When you are ready to grow beyond our pilot suite, we package everything (process, controls, and documentation) into a manufacturing-ready tech transfer. We translate your program into the partner environment, troubleshoot the early runs, and can stay on to manage the relationship through the first commercial campaigns.

Equipment & Methods
  • Tech transfer documentation and control strategy
  • On-site and remote process support
  • Partner selection and qualification support
  • Campaign oversight and troubleshooting
Outputs & Data
  • Manufacturing-ready tech transfer package
  • Batch records and documented process control strategy
  • On-floor support through early campaigns
  • A process that lands, at scale, with quality intact
Exit to commercial scale: the team that engineered the process is the one making sure it lands. We stay involved for as long as you need.
(04) Equipment & Analytics

What We Run It On

An R&D-grade precision fermentation facility specializing in BSL-1 microbial hosts, including downstream processing and analytics that put decision-grade data behind every claim.

Strain & Molecular Biology

  • Cloning and DNA assembly
  • Transformation and clone screening
  • Genotyping and sequence verification
  • Cryo-banking and strain stewardship

Cultivation & Fermentation

  • Microplate and shake-flask cultivation
  • Benchtop controlled bioreactors
  • Pilot bioreactors at 100 L and under
  • Controlled temp, pH, DO, and feed

Downstream Processing

  • Clarification
  • Filtration
  • Purification
  • Drying

Analytical & Characterization

  • Chromatography for titer and purity
  • Spectroscopic characterization
  • Activity and functional assays
  • Identity and quality confirmation

Process Data & Monitoring

  • At-line and in-line monitoring
  • Design-of-experiments workflows
  • Batch records and run logs
  • Trended process performance data

Documentation & Transfer

  • GMP-informed documentation practices
  • Defined process control strategies
  • Tech transfer packages
  • Analytical reports built for review
(05) The Data You Receive

Every Stage Kicks Off Real Data

Whatever point you enter or exit, you leave with defensible, documented data you own. These are the packages a program produces.

Analytical Data Packages

Titer, purity, activity, and characterization results tied to defined methods and an analytical baseline.

Process Documentation

Parameters, control ranges, and batch records that describe exactly how material was made and how to remake it.

Feasibility & Go / No-Go Evidence

An early, honest read on whether a molecule performs and reproduces, with the data to back the decision.

Cost-of-Goods & Scale Models

A transparent cost model and scale-up risk assessment, so there are no surprises when volumes grow.

Strain & Construct Records

Sequence records, construct maps, and strain lineage for a banked, reproducible production host.

Tech Transfer Package

Process, controls, and documentation packaged so a manufacturing partner can pick it up and run.

Know What You Need?

Tell Us Where Your Program Is, and We'll Map the Path

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